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The Santana Room is a location you can visit by using as Santana. If you use Meat Invade as Santana on a player or boss, you'll get teleported to this room until the Meat Invade ends. Once in this room, you'll lose your face and will not be able to exit, unless you reset, or wait until the Meat Invade ends.
The Santana Room is a small box with red walls, most likely to look like the insides of a person.
An easy way to see the room is to use a rokakaka fruit during Meat invade, where you will catch a brief glimpse of the room and yourself in it.